This image shows Michael Heymann

Michael Heymann

Jun.Prof. Dr.

Institute of Biomaterials and Biomolecular Systems
Intelligent Biointegrative Systems

Contact

Pfaffenwaldring 57
70569 Stuttgart
Germany
Room: 9.555

Publications:
  1. Libicher, K., Hornberger, R., Heymann, M., Mutschler, H.: In vitro self-replication and multicistronic expression of large synthetic genomes. Nature Communications. 11, 904 (2020). https://doi.org/10.1038/s41467-020-14694-2.
  2. Knoška, J., Adriano, L., Awel, S., Beyerlein, K.R., Yefanov, O., Oberthuer, D., Peña Murillo, G.E., Roth, N., Sarrou, I., Villanueva-Perez, P., Wiedorn, M.O., Wilde, F., Bajt, S., Chapman, H.N., Heymann, M.: Ultracompact 3D microfluidics for time-resolved structural biology. Nature Communications. 11, 657 (2020). https://doi.org/10.1038/s41467-020-14434-6.
  3. Katzmeier, F., Aufinger, L., Dupin, A., Quintero, J., Lenz, M., Bauer, L., Klumpe, S., Sherpa, D., Dürr, B., Honemann, M., Styazhkin, I., Simmel, F.C., Heymann, M.: A low-cost fluorescence reader for in vitro transcription and nucleic acid detection with Cas13a. PLOS ONE. 14, 1–17 (2019). https://doi.org/10.1371/journal.pone.0220091.
  4. Jensen, S.C., Sullivan, B., Hartzler, D.A., Aguilar, J.M., Awel, S., Bajt, S., Basu, S., Bean, R., Chapman, H.N., Conrad, C., Frank, M., Fromme, R., Martin-Garcia, J.M., Grant, T.D., Heymann, M., Hunter, M.S., Ketawala, G., Kirian, R.A., Knoska, J., Kupitz, C., Li, X., Liang, M., Lisova, S., Mariani, V., Mazalova, V., Messerschmidt, M., Moran, M., Nelson, G., Oberthür, D., Schaffer, A., Sierra, R.G., Vaughn, N., Weierstall, U., Wiedorn, M.O., Xavier, P.L., Yang, J., Yefanov, O., Zatsepin, N.A., Aquila, A., Fromme, P., Boutet, S., Seidler, G.T., Pushkar, Y.: X ‑ ray Emission Spectroscopy at X ‑ ray Free Electron Lasers: Limits to Observation of the Classical Spectroscopic Response for Electronic Structure Analysis. The Journal of Physical Chemistry Letters. 10, 441--446 (2019). https://doi.org/10.1021/acs.jpclett.8b03595.
  5. Hürtgen, D., Mascarenhas, J., Heymann, M., Murray, S.M., Schwille, P., Sourjik, V.: Reconstitution and Coupling of DNA Replication and Segregation in a Biomimetic System. ChemBioChem. 20, 2633–2642 (2019). https://doi.org/10.1002/cbic.201900299.
  6. Yefanov, O., Oberthür, D., Bean, R., Wiedorn, M.O., Knoska, J., Pena, G., Awel, S., Gumprecht, L., Domaracky, M., Sarrou, I., Lourdu Xavier, P., Metz, M., Bajt, S., Mariani, V., Gevorkov, Y., White, T.A., Tolstikova, A., Villanueva-Perez, P., Seuring, C., Aplin, S., Estillore, A.D., Küpper, J., Klyuev, A., Kuhn, M., Laurus, T., Graafsma, H., Monteiro, D.C.F., Trebbin, M., Maia, F.R.N.C., Cruz-Mazo, F., Gañán-Calvo, A.M., Heymann, M., Darmanin, C., Abbey, B., Schmidt, M., Fromme, P., Giewekemeyer, K., Sikorski, M., Graceffa, R., Vagovic, P., Kluyver, T., Bergemann, M., Fangohr, H., Sztuk-Dambietz, J., Hauf, S., Raab, N., Bondar, V., Mancuso, A.P., Chapman, H., Barty, A.: Evaluation of serial crystallographic structure determination within megahertz pulse trains. Structural Dynamics. 6, 064702-- (2019). https://doi.org/10.1063/1.5124387.
  7. Weise, L.I., Heymann, M., Mayr, V., Mutschler, H.: Cell-free expression of RNA encoded genes using MS2 replicase. Nucleic Acids Research. 47, 10956–10967 (2019). https://doi.org/10.1093/nar/gkz817.
  8. Bohne, S., Heymann, M., Chapman, H.N., Trieu, H.K., Bajt, S.: 3D printed nozzles on a silicon fluidic chip. Review of Scientific Instruments. 90, 035108 (2019). https://doi.org/10.1063/1.5080428.
  9. Fuest, M., Schaffer, M., Nocera, G.M., Galilea-Kleinsteuber, R.I., Messling, J.-E., Heymann, M., Plitzko, J.M., Burg, T.P.: In situ Microfluidic Cryofixation for Cryo Focused Ion Beam Milling and Cryo Electron Tomography. Scientific Reports. 9, 19133-- (2019). https://doi.org/10.1038/s41598-019-55413-2.
  10. Eto, H., Soga, N., Franquelim, H.G., Glock, P., Khmelinskaia, A., Kai, L., Heymann, M., Noji, H., Schwille, P.: Design of Sealable Custom-Shaped Cell Mimicries Based on Self-Assembled Monolayers on CYTOP Polymer. ACS Applied Materials & Interfaces. 11, 21372–21380 (2019). https://doi.org/10.1021/acsami.9b05073.
  11. Jia, H., Heymann, M., Härtel, T., Kai, L., Schwille, P.: Temperature-sensitive protein expression in protocells. Chem. Commun. 55, 6421–6424 (2019). https://doi.org/10.1039/C9CC02734C.
  12. Wang, Q., Taschner, M., Ganzinger, K.A., Kelley, C., Villasenor, A., Heymann, M., Schwille, P., Lorentzen, E., Mizuno, N.: Membrane association and remodeling by intraflagellar transport protein IFT172. Nature Communications. 9, 1--13 (2018). https://doi.org/10.1038/s41467-018-07037-9.
  13. Litschel, T., Ramm, B., Maas, R., Heymann, M., Schwille, P.: Beating Vesicles: Encapsulated Protein Oscillations Cause Dynamic Membrane Deformations. Angewandte Chemie International Edition. 57, 16286--16290 (2018). https://doi.org/10.1002/anie.201808750.
  14. Olmos, J.L., Pandey, S., Martin-Garcia, J.M., Calvey, G., Katz, A., Knoska, J., Kupitz, C., Hunter, M.S., Liang, M., Oberthuer, D., Yefanov, O., Wiedorn, M., Heymann, M., Holl, M., Pande, K., Barty, A., Miller, M.D., Stern, S., Roy-Chowdhury, S., Coe, J., Nagaratnam, N., Zook, J., Verburgt, J., Norwood, T., Poudyal, I., Xu, D., Koglin, J., Seaberg, M.H., Zhao, Y., Bajt, S., Grant, T., Mariani, V., Nelson, G., Subramanian, G., Bae, E., Fromme, R., Fung, R., Schwander, P., Frank, M., White, T.A., Weierstall, U., Zatsepin, N., Spence, J., Fromme, P., Chapman, H.N., Pollack, L., Tremblay, L., Ourmazd, A., Phillips, G.N., Schmidt, M.: Enzyme intermediates captured ön the fly" by mix-and-inject serial crystallography. BMC Biology. 16, 1--15 (2018). https://doi.org/10.1186/s12915-018-0524-5.
  15. Haiyang, J., Heymann, M., Bernhard, F., Schwille, P., Kai, L.: Cell free protein synthesis in micro compartments: building a minimal cell from bio bricks. submitted. (2018).
  16. Gicquel, Y., Schubert, R., Kapis, S., Bourenkov, G., Schneider, T., Perbandt, M., Betzel, C., Chapman, H.N., Heymann, M.: Microfluidic chips for in situ crystal X-ray diffraction and in situ dynamic light scattering for serial crystallography. Journal of Visualized Experiments. (2018). https://doi.org/10.3791/57133.
  17. Ramm, B., Glock, P., Mücksch, J., García soriano, D.A., Heymann, M., Schwille, P.: The MinDE system is a generic spatial cue for membrane protein distribution in vitro. Nature Communications. 9, 3942 (2018). https://doi.org/10.1038/s41467-018-06310-1.
  18. Litschel, T., Ganzinger, K.A., Movinkel, T., Heymann, M., Robinson, T., Mutschler, H., Schwille, P.: Freeze-thaw cycles induce content exchange between cell-sized lipid vesicles Freeze-thaw cycles induce content exchange between cell-sized lipid vesicles. New Journal of Physics. 055008, 11 (2018). https://doi.org/10.1088/1367-2630/aabb96.
  19. Jia, H., Kai, L., Heymann, M., Garcia-Soriano, D.A., Härtel, T., Schwille, P.: Light-Induced Printing of Protein Structures on Membranes in Vitro ̈. Nano Letters. 18, 7133--7140 (2018). https://doi.org/10.1021/acs.nanolett.8b03187.
  20. Wiedorn, M.O., Oberthür, D., Bean, R., Schubert, R., Werner, N., Abbey, B., Aepfelbacher, M., Adriano, L., Allahgholi, A., Al-Qudami, N., Andreasson, J., Aplin, S., Awel, S., Ayyer, K., Bajt, S., Barák, I., Bari, S., Bielecki, J., Botha, S., Boukhelef, D., Brehm, W., Brockhauser, S., Cheviakov, I., Coleman, M.A., Cruz-Mazo, F., Danilevski, C., Darmanin, C., Doak, R.B., Domaracky, M., Dörner, K., Du, Y., Fangohr, H., Fleckenstein, H., Frank, M., Fromme, P., Gañán-Calvo, A.M., Gevorkov, Y., Giewekemeyer, K., Ginn, H.M., Graafsma, H., Graceffa, R., Greiffenberg, D., Gumprecht, L., Göttlicher, P., Hajdu, J., Hauf, S., Heymann, M., Holmes, S., Horke, D.A., Hunter, M.S., Imlau, S., Kaukher, A., Kim, Y., Klyuev, A., Knoska, J., Kobe, B., Kuhn, M., Kupitz, C., Küpper, J., Lahey-Rudolph, J.M., Laurus, T., Le Cong, K., Letrun, R., Xavier, P.L., Maia, L., Maia, F.R.N.C., Mariani, V., Messerschmidt, M., Metz, M., Mezza, D., Michelat, T., Mills, G., Monteiro, D.C.F., Morgan, A., Mühlig, K., Munke, A., Münnich, A., Nette, J., Nugent, K.A., Nuguid, T., Orville, A.M., Pandey, S., Pena, G., Villanueva-Perez, P., Poehlsen, J., Previtali, G., Redecke, L., Riekehr, W.M., Rohde, H., Round, A., Safenreiter, T., Sarrou, I., Sato, T., Schmidt, M., Schmitt, B., Schönherr, R., Schulz, J., Sellberg, J.A., Seibert, M.M., Seuring, C., Shelby, M.L., Shoeman, R.L., Sikorski, M., Silenzi, A., Stan, C.A., Shi, X., Stern, S., Sztuk-Dambietz, J., Szuba, J., Tolstikova, A., Trebbin, M., Trunk, U., Vagovic, P., Ve, T., Weinhausen, B., White, T.A., Wrona, K., Xu, C., Yefanov, O., Zatsepin, N., Zhang, J., Perbandt, M., Mancuso, A.P., Betzel, C., Chapman, H., Barty, A.: Megahertz serial crystallography. Nature Communications. 9, 4025 (2018). https://doi.org/10.1038/s41467-018-06156-7.
  21. Niederauer, C., Blumhardt, P., Mücksch, J., Heymann, M., Lambacher, A., Schwille, P.: Direct characterization of the evanescent field in objective-type total internal reflection fluorescence microscopy. Optics Express. 26, 574--582 (2018). https://doi.org/10.1364/OE.26.020492.
  22. Wiedorn, M.O., Awel, S., Morgan, A.J., Ayyer, K., Gevorkov, Y., Fleckenstein, H., Roth, N., Adriano, L., Bean, R., Beyerlein, K.R., Chen, J., Coe, J., Cruz-Mazo, F., Ekeberg, T., Graceffa, R., Heymann, M., Oberthür, D., Samanta, A.K., Sierra, R.G., Stan, C.A., Yefanov, O., Rompotis, D., Correa, J., Erk, B., Treusch, R., Schulz, J., Hogue, B.G., Ganan-Calvo, A.M., Fromme, P., Küpper, J., Rode, A.V., Bajt, S., Kirian, R.A., Chapman, H.N.: Rapid sample delivery for megahertz serial crystallography at X-ray FELs. IUCrJ. 5, 574--584 (2018). https://doi.org/10.1107/S2052252518008369.
  23. Wojtas, D.H., Ayyer, K., Liang, M., Mossou, E., Romoli, F., Seuring, C., Beyerlein, K.R., Bean, R.J., Morgan, A.J., Oberthuer, D., Fleckenstein, H., Heymann, M., Gati, C., Yefanov, O.M., Barthelmess, M., Ornithopoulou, E., Galli, L., Xavier, P.L., Ling, W.L., Frank, M., Yoon, C.H., White, T.A., Bajt, S., Mitraki, A., Boutet, S., Aquila, A., Barty, A., Forsyth, T., Chapman, H.N., Millane, R.P.: Analysis of XFEL serial diffraction data from individual crystalline fibrils. IUCrJ. 4, 795--811 (2017). https://doi.org/10.1107/S2052252517014324.
  24. Jia, H., Heymann, M., Bernhard, F., Schwille, P., Kai, L.: Cell-free protein synthesis in micro compartments: Building a minimal cell from biobricks. New Biotechnology. (2017). https://doi.org/10.1016/j.nbt.2017.06.014.
  25. Rambach, R.W., Linder, K., Heymann, M., Franke, T.: Droplet trapping and fast acoustic release in a multi-height device with steady-state flow. Lab on a Chip. 17, (2017). https://doi.org/10.1039/c7lc00378a.
  26. Beyerlein, K.R., Dierksmeyer, D., Mariani, V., Kuhn, M., Sarrou, I., Ottaviano, A., Awel, S., Knoska, J., Fuglerud, S., Jönsson, O., Stern, S., Wiedorn, M.O., Yefanov, O., Adriano, L., Bean, R., Burkhardt, A., Fischer, P., Heymann, M., Horke, D.A., Jungnickel, K.E.J., Kovaleva, E., Lorbeer, O., Metz, M., Meyer, J., Morgan, A., Pande, K., Panneerselvam, S., Seuring, C., Tolstikova, A., Lieske, J., Aplin, S., Roessle, M., White, T.A., Chapman, H.N., Meents, A., Oberthuer, D.: Mix-and-diffuse serial synchrotron crystallography. IUCrJ. 4, (2017). https://doi.org/10.1107/S2052252517013124.
  27. Wiedorn, M.O., Awel, S., Morgan, A.J., Barthelmess, M., Bean, R., Beyerlein, K.R., Chavas, L.M.G., Eckerskorn, N., Fleckenstein, H., Heymann, M., Horke, D.A., Knoska, J., Mariani, V., Oberthür, D., Roth, N., Yefanov, O., Barty, A., Bajt, S., Küpper, J., Rode, A.V., Kirian, R.A., Chapman, H.N.: Post-sample aperture for low background diffraction experiments at X-ray free-electron lasers. Journal of Synchrotron Radiation. 24, (2017). https://doi.org/10.1107/S1600577517011961.
  28. Oberthuer, D., Knoska, J., Wiedorn, M.O., Beyerlein, K.R., Bushnell, D.A., Kovaleva, E.G., Heymann, M., Gumprecht, L., Kirian, R.A., Barty, A., Mariani, V., Tolstikova, A., Adriano, L., Awel, S., Barthelmess, M., Dörner, K., Xavier, P.L., Yefanov, O.M., James, D., Nelson, G., Wang, D., Calvey, G., Chen, Y., Schmidt, A., Szczepek, M., Frielingsdorf, S., Lenz, O., Snell, E., Robinson, P.J., Sarler, B., Belsak, G., Macek, M., Wilde, F., Aquila, A., Boutet, S., Liang, M., Hunter, M.S., Scheerer, P., Lipscomb, J.D., Weierstall, U., Kornberg, R.D., Spence, J.C.H., Pollack, L., Chapman, H.N., Bajt, S.: Double-flow focused liquid injector for efficient serial femtosecond crystallography. Scientific Reports. 7, 44628 (2017). https://doi.org/10.1038/srep44628.
  29. Aghvami, S.A., Opathalage, A., Zhang, Z.K., Ludwig, M., Heymann, M., Norton, M., Wilkins, N., Fraden, S.: Rapid prototyping of cyclic olefin copolymer (COC) microfluidic devices. Sensors and Actuators, B: Chemical. 247, (2017). https://doi.org/10.1016/j.snb.2017.03.023.
  30. Schubert, R., Kapis, S., Gicquel, Y., Bourenkov, G., Schneider, T.R.T.R., Heymann, M., Betzel, C., Perbandt, M.: A multicrystal diffraction data-collection approach for studying structural dynamics with millisecond temporal resolution. IUCrJ. 3, 393--401 (2016). https://doi.org/10.1107/S2052252516016304.
  31. Nelson, G., Kirian, R.A.R.A., Weierstall, U., Zatsepin, N.A., Faragó, T., Baumbach, T., Wilde, F., Niesler, F.B.P., Zimmer, B., Ishigami, I., Hikita, M., Bajt, S., Yeh, S.-R., Rousseau, D.L., Chapman, H.N., Spence, J.C.H., Heymann, M.: Three-dimensional-printed gas dynamic virtual nozzles for x-ray laser sample delivery. Optics Express. 24, 11515--11530 (2016). https://doi.org/10.1364/OE.24.011515.
  32. Wang, A.L.L., Gold, J.M.M., Tompkins, N., Heymann, M., Harrington, K.I.I., Fraden, S.: Configurable NOR gate arrays from. European Physical Journal. 225, 211--227 (2016). https://doi.org/10.1140/epjst/e2016-02622-y.
  33. Chen, D.T.N., Heymann, M., Fraden, S., Nicastro, D., Dogic, Z.: ATP Consumption of Eukaryotic Flagella Measured at a Single-Cell Level. Biophysical Journal. 109, 2562--2573 (2015). https://doi.org/10.1016/j.bpj.2015.11.003.
  34. Tompkins, N., Cambria, M.C., Wang, A.L., Heymann, M., Fraden, S.: Creation and perturbation of planar networks of chemical oscillators. Chaos. 25, 064611 (2015). https://doi.org/10.1063/1.4922056.
  35. Kirian, R.A., Awel, S., Eckerskorn, N., Fleckenstein, H., Wiedorn, M., Adriano, L., Bajt, S., Barthelmess, M., Bean, R., Beyerlein, K.R., Chavas, L.M.G., Domaracky, M., Heymann, M., Horke, D. a., Knoska, J., Metz, M., Morgan, A., Oberthuer, D., Roth, N., Sato, T., Xavier, P.L., Yefanov, O., Rode, a V., Küpper, J., Chapman, H.N.: Simple convergent-nozzle aerosol injector for single-particle diffractive imaging with X-ray free-electron lasers. Structural Dynamics. 2, 041717 (2015). https://doi.org/10.1063/1.4922648.
  36. Beyerlein, K.R., Adriano, L., Heymann, M., Kirian, R.A., Knoska, J., Wilde, F., Chapman, H.N., Knoska, J., Wilde, F., Chapman, H.N., Bajt, S.: Ceramic micro-injection molded nozzles for serial femtosecond crystallography sample delivery. Review of Scientific Instruments. 86, 125104 (2015). https://doi.org/10.1063/1.4936843.
  37. Feld, G.K., Heymann, M., Benner, W.H., Pardini, T., Tsai, C.-J., Boutet, S., Coleman, M.A., Hunter, M.S., Li, X.-D.X., Messerschmidt, M., Opthalage, A., Pedrini, B., Williams, G.J., Krantz, B.A., Fraden, S., Hau-Riege, S.P., Evans, J.E., Segelke, B.W., Frank, M., Opathalage, A., Pedrini, B., Williams, G.J., Krantz, B.A., Fraden, S., Hau-Riege, S.P., Evans, J.E., Segelke, B.W., Frank, M.: Low- Z polymer sample supports for fixed-target serial femtosecond X-ray crystallography. Journal of Applied Crystallography. 48, 1072--1079 (2015). https://doi.org/10.1107/S1600576715010493.
  38. Nogly, P., James, D., Wang, D., White, T. a., Zatsepin, N., Shilova, A., Nelson, G., Liu, H., Johansson, L., Heymann, M., Jaeger, K., Metz, M., Wickstrand, C., Wu, W., B\aath, P., Berntsen, P., Oberthuer, D., Panneels, V., Cherezov, V., Chapman, H., Schertler, G., Neutze, R., Spence, J., Moraes, I., Burghammer, M., Standfuss, J., Weierstall, U.: Lipidic cubic phase serial millisecond crystallography using synchrotron radiation. IUCrJ. 2, 168--176 (2015). https://doi.org/10.1107/S2052252514026487.
  39. Tenboer, J., Basu, S., Zatsepin, N., Pande, K., Milathianaki, D., Frank, M., Hunter, M., Boutet, S., Williams, G.J., Koglin, J.E., Oberthuer, D., Heymann, M., Kupitz, C., Conrad, C., Coe, J., Roy-Chowdhury, S., Weierstall, U., James, D., Wang, D., Grant, T., Barty, A., Yefanov, O., Scales, J., Gati, C., Seuring, C., Srajer, V., Henning, R., Schwander, P., Fromme, R., Ourmazd, A., Moffat, K., Van Thor, J.J., Spence, J.C.H., Fromme, P., Chapman, H.N., Schmidt, M.: Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein. Science. 346, 1242--1246 (2014). https://doi.org/10.1126/science.1259357.
  40. Tompkins, N., Li, N., Girabawe, C., Heymann, M., Ermentrout, G.B., Epstein, I.R., Fraden, S.: Testing Turing’s theory of morphogenesis in chemical cells. PNAS. 111, 4397--402 (2014). https://doi.org/10.1073/pnas.1322005111.
  41. Kornreich, M., Heymann, M., Fraden, S., Beck, R.: Cross polarization compatible dialysis chip. Lab on a chip. 14, 3700--4 (2014). https://doi.org/10.1039/c4lc00600c.
  42. Akella, S.V., Mowitz, A., Heymann, M., Fraden, S.: Emulsion-Based Technique To Measure Protein Crystal Nucleation Rates of Lysozyme. Crystal Growth and Design. 14, 4487--4509 (2014). https://doi.org/10.1021/cg500562r.
  43. Heymann, M., Opthalage, A., Wierman, J.L., Akella, S., Szebenyi, D.M.E., Gruner, S.M., Fraden, S.: Room-temperature serial crystallography using a kinetically optimized microfluidic device for protein crystallization and on-chip X-ray diffraction. IUCrJ. 1, 349--360 (2014). https://doi.org/10.1107/S2052252514016960.
  44. Heymann, M., Fraden, S., Kim, D.: Multi-Height Precision Alignment With Selectively Developed Alignment Marks. Journal of Microelectromechanical Systems. 23, 1--4 (2013). https://doi.org/10.1109/JMEMS.2013.2279231.
  45. Sanchez, T., Chen, D.T.N., DeCamp, S.J., Heymann, M., Dogic, Z.: Spontaneous motion in hierarchically assembled active matter. Nature. 491, 431--4 (2012). https://doi.org/10.1038/nature11591.
  46. Heymann, M., Harrington, K.I., Pollack, J., Fraden, S.: En Route to Signal Inversion in Chemical Computing. Proceedings of Artificial Life XII. 166--167 (2010).
  47. Romanowsky, M.B., Heymann, M., Abate, A.R., Krummel, A.T., Fraden, S., Weitz, D.A.: Functional patterning of PDMS microfluidic devices using integrated chemo-masks. Lab on a chip. 10, 1521--4 (2010). https://doi.org/10.1039/c004050a.
To the top of the page