Bioanalytics I

Biophysics Group / Nussberger Lab

The qualitative and quantitative tracking of biological processes at the molecular level requires direct or indirect detection of biomolecules, such as proteins, nucleic acids or metabolites. This lecture deals with the basic bioanalytical methdods that are used and applied in almost every life sciences laboratory. This lecture is designed for students in the bachelor's program Technical Biology.

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C@MPUS

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ILIAS

General Information

Lecturer

Prof. Dr. Dieter Jendrossek and Prof. Dr. Stephan Nussberger

Term

Summer

Credits

6 ETCS

Language

German

Schedule

Thursdays 15:30 - 17:00 Uhr / Allmand. 31 0.106

  • The next lecture will take place again in the summer term 2023.

Main topics

  • Topic 1: Separation and detection techniques of biomolecules
    • Protein determination methods: A280 / A205, fluorescence, biuret (Lowry, Beisenherz, bicinchoninic acid) and Bradford (Jendrossek)
    • Basics of Enzyme Kinetics (Jendrossek)
    • Protein cleavage methods (Jendrossek)
    • Chromatographic separation methods: chromatofocusing, hydroxapatite chromatography, reversed phase chromatography, hydrophobic interaction chromatography, thin layer chromatography, affinity chromatography (Ni-NTA, MBP, glutathione-S-transferase, PHB, chitin, cellulase, avidin / biotin, intein and streptag (Jendrossek)
    • Electrophoretic separation and detection methods: Southern blot, Northern blot, Western blot, Capillary electrophoresis (Jendrossek)
  • Topic 2: Mass spectrometric methods
    • Matrix Assisted Laser Desorption Ionization Mass Spectrometry (MALDI) (Jendrossek)
    • Electrospray Ionization Mass Spectrometry (ESI) (Jendrossek)
  • Topic 3: Optical concentration and structure analysis
    • UV / VIS techniques in the bioanalytics of proteins and nucleic acids (Nussberger)
    • Circular dichrosimus and optical rotation dispersion for secondary structure analysis of proteins and chiral recognition of molecules (ORD, CD) (Nussberger)
    • Fluorescence-based bioanalytics (Nussberger)
    • Infrared spectroscopy (IR, FTIR, Raman) (Nussberger)
Informations
  • An up-to-date overview with lecture dates can be found at C @ MPUS
  • All documents are available for download in the ILIAS course.
Literature
  • Lottspeich, Engels (Hrsg.) Bioanalytik (Springer Spektrum) 
  • for reference, see C@MPUS
  • Successful participation requires regular participation in the lecture.
  • In addition, regular participation in the internship Bioanalytik I is expected.

Contact

 

Biophysics Group, Institute of Biomaterials and biomolecular Systems, University of Stuttgart

Pfaffenwaldring 57 , D-70569 Stuttgart, Germany

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