Biophysics Group / Nussberger Lab

Bioanalytics II

Modern bioanalytics contributes to the solution of challenging tasks in different areas of life science. Important tools include classical techniques such as x-ray crystallography and cryo-electron microscopy for the three-dimensional structure determination of large macromolecular complexes, super-resolution microscopy for the resolution of objects below the diffraction limit of 200 nm, and nano-pore arrays for high-resolution single-molecule analysis. This lecture series deals with these topics; it is designed for students in the master's programs in Technical Biology and Biomedical Engineering.

General Information

Lecturer

Prof. Dr. Stephan Nussberger and Prof. Dr. Dieter Jendrossek

Term

Winter

Credits

12 ETCS

Language

German or English

Schedule

Thursdays 9:45 - 11:15 h / PWR 57 V9.330

  • The next lecture will take place in the winter semester 2020/2021.
  • First lecture: TBA

Main Topics

  • Topic 1: Fundamentals of X-ray crystallography 
    • Bravais grid, Fourier theory (Nussberger)
    • Ewald construction (Nussberger) 
    • Solution of the phase problem (Nussberger)
  • Topic 2: Basics of Cryo-Transmission Electron Microscopy (Nussberger)
  • Topic 3: Super-resolution microscopy 
    • Near-field optical microscopy and total internal reflection fluorescence microscopy (TIRFM) (Nussberger)
    • Stochastic Optical Reconstruction Microscopy (STORM) and Photoactivated Localization Microscopy (PALM) (Nussberger)
    • Stimulated Emission Depletion Microscopy (Nussberger)
  • Topic 4: Methods for manipulating individual molecules
    • Optical tweezers (Nussberger)
    • Scanning Probe Microscopy (AFM, SNOM) (Nussberger)
  • Topic 5: Electrophysiological Methods
    • Patch Clamp (Nussberger)
    • Single Molecule DNA Sequencing (Nussberger)
    • Droplet hydrogel bilayer (Nussberger)
  • Topic 6: Genetic analysis
    • 2-hybrid system (Jendrossek)
    • DNA chips (Jendrossek)
    • Directed mutagenesis (Jendrossek)
  • An up-to-date overview with lecture dates can be found in CAMPUS.
  • All documents are available for download in the ILIAS course.
Literature
  • Bernhard Rupp, Biomolecular Crystallography: Principles, Practice, and Application to Structural Biology (Garland, 2010)
  • Paul R. Selvin and Taekjip Ha, Single-Molecule Techniques (Cold Spring Harbor Laboratory Press 2008)
  • Lottspeich, Engels (Hrsg.) Bioanalytik (Springer Spektrum) 
  • Joseph R. Lakowicz, Principles of Fluorescence Spectroscopy (Springer, 2006) 
  • Seminar paper and lecture on a topic of molecular bioanalytics
  • Submission of the seminar paper and date of the lecture: End of the semester
  • Successful participation in the lab course 

Contact

 

Biophysics Group, Institute of Biomaterials and biomolecular Systems, University of Stuttgart

Pfaffenwaldring 57 , D-70569 Stuttgart, Germany

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