Contact
+49 711 685 65075
+49 711 685 65096
Email
Pfaffenwaldring 57
70569 Stuttgart
Deutschland
Room: 9-552
Subject
Geminivirus-host plant interaction
Interactions between viral and plant proteins are essential for reprogramming cellular processes and thus for a successful viral life cycle. At the same time, they provide targets for the development of new, customized, anti-viral methods.
Main focus: Investigation of the cell biological and molecular basis of intra- and intercellular viral transport and pathogenicity as well as plant macromolecular transport processes by analyzing:
- Viral exploitation and induced restructuring of cell structures (e.g. cytoskeleton and endomembrane system)
- Protein-protein interactions (virus-host cell & virus-virus)
- Regulation of the multifunctionality of viral porters by post-translational modification (e.g. phosphorylation)
Phytoviral Diagnostics
Molecular tools for monitoring and identification of plant viruses.
Main focus: Nucleic acid-based detection of plant viruses by isothermal amplification, e.g. phi29-based rolling circle amplification (RCA) of circular viral DNA and restriction fragment length polymorphism (RFLP) analysis.
Phytoviral Engineering
Utilization of plant virus genomes and their highly efficient regulatory elements for vector systems.
Main focus: Plant virus-based expression and silencing vectors for biotechnology & functional genomic analysis, including heterologous expression of scaffold proteins for nanostructured 3D-printed tissue equivalents and of therapeutic antibodies.
(As corresponding author in bold)
Happle, A., Jeske, H., Kleinow, T. (2021) Dynamic subcellular distribution of begomoviral nuclear shuttle and movement proteins. Virology, 562, 158-175. DOI: 10.1016/j.virol.2021.07.014
Berking, T., Lorenz, S.G., Ulrich, A.B., Greiner, J., Kervio, E., Bremer, J., Wege, C., Kleinow, T., Richert, C. (2021). The effect of pooling on the detection of the nucleocapsid protein of SARS-CoV-2 with rapid antigen tests. Diagnostics, 11, 1290. DOI: 10.3390/diagnostics11071290
Hasanvand, V., Heydarnejad, J., Massumi, H., Kleinow, T., Jeske, H., Fontenele, R.S., Kraberger, S., Varsani A. (2021) Genome characterization of parsley severe stunt-associated virus in Iran. Virus Genes, 57, 293-301. DOI: 10.1007/s11262-021-01835-y
Kleinow, T., Happle, A., Kober, S., Linzmeier, L., Rehm, T.M., Fritze, J., Buchholz, P.C.F., Kepp, G., Jeske, H., Wege, C. (2020) Phosphorylations of the Abutilon mosaic virus movement protein affect its self-interaction, symptom development, viral DNA accumulation and host range. Front. Plant Sci., 11, 1155. DOI: 10.3389/fpls.2020.01155
Hasanvand, V., Heydarnejad, J., Massumi, H., Kleinow, T., Jeske, H. (2020) First report of parsley severe stunt associated virus in Iran. New Disease Reports, 41, 17. DOI: dx.doi.org/10.5197/j.2044-0588.2020.041.017
Hasanvand, V., Heydanejad, J., Massumi, H., Kleinow, T., Jeske, H., Varsani, A. (2020) Isolation and characterization of a novel geminivirus from parsley. Virus Res., 286, 198056. DOI: 10.1016/j.virusres.2020.198056
Hipp, K., Zikeli, K., Kepp, G., Schmid, L., Shoeman, R.L., Jurkowskic, T.P., Kleinow, T., Jeske, H. (2019) Different forms of African cassava mosaic virus capsid protein within plants and virions. Virology, 529, 81–90. DOI: 10.1016/j.virol.2019.01.018
Krapp, S., Schuy, C., Greiner, E., Stephan, I., Alberter, B., Funk, C., Marschall, M., Wege, C., Bailer, S., Kleinow, T., Krenz, B. (2017) Begomoviral movement protein effects in human and plant cells: towards new potential interaction partners . Viruses , 9, 334. DOI: 10.3390/v9110334
Kleinow, T. (ed.) (2016) Plant-Virus Interactions: Molecular Biology, Intra- and Intercellular Transport. Springer International Publishing Switzerland, DOI: 10.1007/978-3-319-25489-0_1
Krenz, B., Deuschle, K., Deigner, T., Unseld, S., Kepp, G., Wege, C., Kleinow, T., Jeske, H. (2015). Early functions of the Abutilon mosaic virus AC2 gene as a replication brake. J. Virol., 89, 3683-3699. DOI: 10.1128/JVI.03491-14
Krenz, B., Guo, T.W., Kleinow, T. (2014) Stromuling when stressed! Acta Soc. Bot. Pol., 83, 325-329. DOI: 10.5586/asbp.2014.050
Richter, K., Kleinow, T., Jeske, H. (2014). Somatic homologous recombination in plants is promoted by a geminivirus in a tissue-selective manner. Virology, 452, 287-296. DOI: 10.1016/j.virol.2014.01.024
Krenz, B., Jeske, H., Kleinow, T. (2012). The induction of stromule formation by a plant DNA-virus in epidermal leaf tissues suggests a novel intra- and intercellular macromolecular trafficking route. Front. Plant Sci., 3, 291. DOI: 10.3389/fpls.2012.00291
Krenz, B., Neugart, F., Kleinow, T., Jeske, H. (2011). Self-interaction of Abutilon mosaic virus replication initiator protein (Rep) in plant cell nuclei. Virus Res., 161, 194-197. DOI: 10.1016/j.virusres.2011.07.02
Krenz, B., Windeisen, V., Wege, C., Jeske, H., Kleinow, T. (2010). A plastid-targeted heat shock cognate 70 kDa protein interacts with the Abutilon mosaic virus movement protein. Virology, 401, 6-17. DOI: 10.1016/j.virol.2010.02.011
Kleinow, T., Tanwir, F., Kocher, C., Krenz, B., Wege, C., Jeske, H. (2009c). Expression dynamics and ultrastructural localization of epitope-tagged Abutilon mosaic virus nuclear shuttle and movement proteins in Nicotiana benthamiana cells. Virology, 391, 212-220. DOI: 10.1016/j.virol.2009.06.042
Kleinow, T., Himbert, S., Krenz, B., Jeske, H., Koncz, C. (2009b). NAC domain transcription factor ATAF1 interacts with SNF1-related kinases and silencing of its subfamily causes severe developmental defects in Arabidopsis. Plant Sci., 177, 360-370. DOI: 10.1016/j.plantsci.2009.06.011
Kleinow, T., Nischang, M., Beck, A., Kratzer, U., Tanwir, F., Preiss, W., Kepp, G., Jeske, H. (2009a). Three C-terminal phosphorylation sites in the Abutilon mosaic virus movement protein affect symptom development and viral DNA accumulation. Virology, 390, 89-101. DOI: 10.1016/j.virol.2009.04.018
Kleinow, T., Holeiter, G., Nischang, M., Stein, M., Karayavuz, M., Wege, C., Jeske, H. (2008). Post-translational modifications of Abutilon mosaic virus movement protein (BC1) in fission yeast. Virus Res., 131, 86-94. DOI: 10.1016/j.virusres.2007.08.01
Frischmuth, S., Kleinow, T., Aberle, H.J., Wege, C., Hülser, D., Jeske, H. (2004). Yeast two-hybrid systems confirm the membrane-association and oligomerization of BC1 but do not detect an interaction of the movement proteins BC1 and BV1 of Abutilon mosaic geminivirus. Arch. Virol., 149, 2349-2364. DOI: 10.1007/s00705-004-0381-0
Rios, G., Lossow, A., Hertel, B., Breuer, F., Schaefer, S., Broich, M., Kleinow, T., Jasik, J., Winter, J., Ferrando, A., Farras, R., Panicot, M., Henriques, R., Mariaux, JB., Oberschall, A., Molnar, G., Berendzen, K., Shukla, V., Lafos, M., Koncz, Z., Redei, G.P., Schell, J., Koncz, C. (2002) Rapid identification of Arabidopsis insertion mutants by non-radioactive detection of T-DNA tagged genes. Plant J., 32, 243-253. DOI: 10.1046/j.1365-313x.2002.01416.x
Farras, R., Ferrando, A., Jasik, J., Kleinow, T., Okresz, L., Tiburcio, A., Salchert, K., del Pozo, C., Schell, J., Koncz, C. (2001) SKP1-SnRK protein kinase interactions mediate proteasomal binding of a plant SCF ubiquitin ligase. EMBO J., 20, 2742-2756. DOI: 10.1093/emboj/20.11.2742
Lumbreras, V., Alba, M.M., Kleinow, T., Koncz, C., Pages, M. (2001) Domain fusion between SNF1-related kinase subunits during plant evolution. EMBO Rep., 2, 55-60. DOI: 10.1093/embo-reports/kve001
Kleinow, T., Bhalerao, R., Breuer, F., Umeda, M., Salchert, K., Koncz, C. (2000) Functional identification of an Arabidopsis snf4 ortholog by screening for heterologous multicopy suppressors of snf4 deficiency in yeast. Plant J., 23, 115-122. DOI: 10.1046/j.1365-313x.2000.00809.x
Shell, J., Bisseling, T., Dülz, M., Franssen, H., Fritze, K., John, M., Kleinow, T., Leßnik, A., Miklahevichs, E., Pawloski, K., Röhrig, H., van de Sande, K., Schmidt, J., Steinbiß, H.H., Stoll, M. (1999) Re-evaluation of phytohormone-independent division of tobacco protoplast-derived cells. Plant J., 17, 461-466. DOI: 10.1046/j.1365-313X.1999.00404.x
Kellmann, J.W., Kleinow, T., Engelhardt, K., Philipp, C., Wegener, D., Schell, J., Schreier, P.H. (1996) Characterization of two class II chitinase genes from peanut and expression studies in transgenic tobacco plants. Plant Mol. Biol., 30, 351-358. DOI: 10.1007/BF00020121
B.Sc. Technical Biology
Lecture "Molecular Biology" + associated laboratory exercise " Insights into molecular biological analysis" Module Technical Biology III B.Sc. Technical Biology 3rd semester and minor subjects
Laboratory exercise + accompanying seminar "Molecular biology from theory to experiment and vice versa" compulsory elective module Biological Practice Technical Biology (B.Sc. in 5th semester)
Module Coordinator "Learning through teaching" and "Be a tutor: Support the start into the technical biology studies", subject-related key qualification B.Sc. technical biology; implementation of preparatory didactic seminar, especially for first semester tutors
Examiner & Supervisor Scientific work & theses B.Sc. Technical Biology, Theses to be offered on various topics: Geminivirus-host plant interaction, development of phytoviral diagnostics and phytoviral engineering (details under subject area)
M.Sc. Technical Biology
Lecture "Bionanotechnology, functional materials and molecular biology with plant viruses", specialization subject biomaterials and nanobiotechnology M.Sc. Technical Biology
Seminar lecture + corresponding exercise " Scientific Methodology II" specialization module M.Sc. Technical Biology
Journal Club Molecular Biology (literature seminar in English) subject related key qualifications M.Sc. Technical Biology
Examiner & Supervisor Project study & thesis M.Sc. Technical Biology, Theses to be offered on various topics: Geminivirus-host plant interaction, development of phytoviral diagnostics and phytoviral engineering (details under subject area)
B.Sc. Food Chemistry
Laboratory practical exercise "Biology for Food Chemists" B.Sc. Food Chemistry 3rd semester
Lecture "Molecular Biology" B.Sc. Food Chemistry 3rd semester
| Since 2002 |
Project leader "Geminivirus-host plant interaction: transport and pathogenicity", phytoviral engineering (virus-based vector systems for functional genome analysis & plant-based expression), phytoviral diagnostics Institute of Biomaterials and Biomolecular Systems Since 10/2019 Head of Molecular Biology Support Unit until 09/2019 Dept. of Molecular Biology and Virology of Plants |
| 01/2011 |
Privatdozent; Habilitation Venia Legendi " Molecular Biology and Virology of Plants". |
| 2007 |
Advanced training in higher education didactics, acquisition of the "Baden-Württemberg Certificate for Higher Education Didactics" Regional Association for Higher Education Didactics, Stuttgart |
| 2000 - 2002 |
Post-doctoral researcher; project "Characterization of SNF1-like kinase complex in stress and sugar signal transduction of Arabidopsis". Max Planck Institute for Plant Breeding Research, Dept. of Genetic Basics of Plant Breeding, Cologne, Germany |
| 07/2000 |
PhD (Dr. rer. nat.), University of Cologne; Project "Identification and characterization of potential components of stress and glucose signal transduction" |
| 1996 - 2000 |
Research Associate Max Planck Institute for Plant Breeding Research, Dept. of Genetic Basics of Plant Breeding, Cologne, Germany |
| 1990 - 1996 |
1990 - 1996 Biology studies (Diploma), University of Cologne |
Committee activities and Responsibilities
- Senate Committee on Study Abroad and Grants, Representative of Academic Staff
- Senate Committee for Diversity and Equality, representative of the academic staff
- Examination Committee Technical Biology, representative of the academic staff
- Admissions Committee for the B.Sc. program in Technical Biology, representative of the academic staff
- Equal Opportunity Officer / Deputy Equal Opportunity Officer of Faculty 4, University of Stuttgart
- Project leader for genetic engineering work (S1 & S2) Molecular Biology and Virology of Plants
- Radiation protection officer of the Institute of Biomaterials and Biomolecular Systems
Editor & Honorary activities
- Review Editor for Technical Advances in Plant Science; Frontiers in Plant Science
- Editor New Disease Reports
- 2011 - 2018 Honorary activity as head of the working group "Virus Diseases of Plants" German Society for Plant Protection and Plant Health r. S. (DPG)