27. Juni 2019 /

Cryo-EM structure of Neurospora crassa respiratory complex IV: Bausewein et al. (2019) IUCrJ 6: 773-780, coauthored by S. Nussberger / Biophysics group


In fungi, the mitochondrial respiratory chain complexes (complexes I–IV) are responsible for oxidative phosphorylation, as in higher eukaryotes. Cryo-EM was used to identify a 200 kDa membrane protein from Neurospora crassa in lipid nanodiscs as cytochrome c oxidase (complex IV) and its structure was determined at 5.5 Å resolution. The map closely resembles the cryo-EM structure of complex IV from Saccharomyces cerevisiae. Its ten subunits are conserved in S. cerevisiae and Bos taurus, but other transmembrane subunits are missing. The different structure of the Cox5a subunit is typical for fungal complex IV and may affect the interaction with complex III in a respiratory supercomplex. Additional density was found between the matrix domains of the Cox4 and Cox5a subunits that appears to be specific to N. crassa.

For reference, see   Cryo-EM structure of the N. crassa respiratory complex IV. Bausewein, T., Nussberger, S. & Kühlbrandt, W.  IUCrJ 6: 773-780  (2019)



Abteilung Biophysik, Institut für Biomaterialien und biomolekulare Systeme, Universität Stuttgart

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