Publikationen

Veröffentlichungen der Arbeitsgruppe Intelligente Biointegrative Systeme

  1. 2020

    1. Libicher, K., Hornberger, R., Heymann, M., Mutschler, H.: In vitro self-replication and multicistronic expression of large synthetic genomes. Nature Communications. 11, 904 (2020). https://doi.org/10.1038/s41467-020-14694-2.
    2. Knoška, J., Adriano, L., Awel, S., Beyerlein, K.R., Yefanov, O., Oberthuer, D., Peña Murillo, G.E., Roth, N., Sarrou, I., Villanueva-Perez, P., Wiedorn, M.O., Wilde, F., Bajt, S., Chapman, H.N., Heymann, M.: Ultracompact 3D microfluidics for time-resolved structural biology. Nature Communications. 11, 657 (2020). https://doi.org/10.1038/s41467-020-14434-6.
  2. 2019

    1. Weise, L.I., Heymann, M., Mayr, V., Mutschler, H.: Cell-free expression of RNA encoded genes using MS2 replicase. Nucleic Acids Research. 47, 10956–10967 (2019). https://doi.org/10.1093/nar/gkz817.
    2. Bohne, S., Heymann, M., Chapman, H.N., Trieu, H.K., Bajt, S.: 3D printed nozzles on a silicon fluidic chip. Review of Scientific Instruments. 90, 035108 (2019). https://doi.org/10.1063/1.5080428.
    3. Hürtgen, D., Mascarenhas, J., Heymann, M., Murray, S.M., Schwille, P., Sourjik, V.: Reconstitution and Coupling of DNA Replication and Segregation in a Biomimetic System. ChemBioChem. 20, 2633–2642 (2019). https://doi.org/10.1002/cbic.201900299.
    4. Katzmeier, F., Aufinger, L., Dupin, A., Quintero, J., Lenz, M., Bauer, L., Klumpe, S., Sherpa, D., Dürr, B., Honemann, M., Styazhkin, I., Simmel, F.C., Heymann, M.: A low-cost fluorescence reader for in vitro transcription and nucleic acid detection with Cas13a. PLOS ONE. 14, 1–17 (2019). https://doi.org/10.1371/journal.pone.0220091.
    5. Fuest, M., Schaffer, M., Nocera, G.M., Galilea-Kleinsteuber, R.I., Messling, J.-E., Heymann, M., Plitzko, J.M., Burg, T.P.: In situ Microfluidic Cryofixation for Cryo Focused Ion Beam Milling and Cryo Electron Tomography. Scientific Reports. 9, 19133-- (2019). https://doi.org/10.1038/s41598-019-55413-2.
    6. Eto, H., Soga, N., Franquelim, H.G., Glock, P., Khmelinskaia, A., Kai, L., Heymann, M., Noji, H., Schwille, P.: Design of Sealable Custom-Shaped Cell Mimicries Based on Self-Assembled Monolayers on CYTOP Polymer. ACS Applied Materials & Interfaces. 11, 21372–21380 (2019). https://doi.org/10.1021/acsami.9b05073.
    7. Yefanov, O., Oberthür, D., Bean, R., Wiedorn, M.O., Knoska, J., Pena, G., Awel, S., Gumprecht, L., Domaracky, M., Sarrou, I., Lourdu Xavier, P., Metz, M., Bajt, S., Mariani, V., Gevorkov, Y., White, T.A., Tolstikova, A., Villanueva-Perez, P., Seuring, C., Aplin, S., Estillore, A.D., Küpper, J., Klyuev, A., Kuhn, M., Laurus, T., Graafsma, H., Monteiro, D.C.F., Trebbin, M., Maia, F.R.N.C., Cruz-Mazo, F., Gañán-Calvo, A.M., Heymann, M., Darmanin, C., Abbey, B., Schmidt, M., Fromme, P., Giewekemeyer, K., Sikorski, M., Graceffa, R., Vagovic, P., Kluyver, T., Bergemann, M., Fangohr, H., Sztuk-Dambietz, J., Hauf, S., Raab, N., Bondar, V., Mancuso, A.P., Chapman, H., Barty, A.: Evaluation of serial crystallographic structure determination within megahertz pulse trains. Structural Dynamics. 6, 064702-- (2019). https://doi.org/10.1063/1.5124387.
    8. Jia, H., Heymann, M., Härtel, T., Kai, L., Schwille, P.: Temperature-sensitive protein expression in protocells. Chem. Commun. 55, 6421–6424 (2019). https://doi.org/10.1039/C9CC02734C.
    9. Jensen, S.C., Sullivan, B., Hartzler, D.A., Aguilar, J.M., Awel, S., Bajt, S., Basu, S., Bean, R., Chapman, H.N., Conrad, C., Frank, M., Fromme, R., Martin-Garcia, J.M., Grant, T.D., Heymann, M., Hunter, M.S., Ketawala, G., Kirian, R.A., Knoska, J., Kupitz, C., Li, X., Liang, M., Lisova, S., Mariani, V., Mazalova, V., Messerschmidt, M., Moran, M., Nelson, G., Oberthür, D., Schaffer, A., Sierra, R.G., Vaughn, N., Weierstall, U., Wiedorn, M.O., Xavier, P.L., Yang, J., Yefanov, O., Zatsepin, N.A., Aquila, A., Fromme, P., Boutet, S., Seidler, G.T., Pushkar, Y.: X ‑ ray Emission Spectroscopy at X ‑ ray Free Electron Lasers: Limits to Observation of the Classical Spectroscopic Response for Electronic Structure Analysis. The Journal of Physical Chemistry Letters. 10, 441--446 (2019). https://doi.org/10.1021/acs.jpclett.8b03595.
  3. 2018

    1. Olmos, J.L., Pandey, S., Martin-Garcia, J.M., Calvey, G., Katz, A., Knoska, J., Kupitz, C., Hunter, M.S., Liang, M., Oberthuer, D., Yefanov, O., Wiedorn, M., Heymann, M., Holl, M., Pande, K., Barty, A., Miller, M.D., Stern, S., Roy-Chowdhury, S., Coe, J., Nagaratnam, N., Zook, J., Verburgt, J., Norwood, T., Poudyal, I., Xu, D., Koglin, J., Seaberg, M.H., Zhao, Y., Bajt, S., Grant, T., Mariani, V., Nelson, G., Subramanian, G., Bae, E., Fromme, R., Fung, R., Schwander, P., Frank, M., White, T.A., Weierstall, U., Zatsepin, N., Spence, J., Fromme, P., Chapman, H.N., Pollack, L., Tremblay, L., Ourmazd, A., Phillips, G.N., Schmidt, M.: Enzyme intermediates captured ön the fly" by mix-and-inject serial crystallography. BMC Biology. 16, 1--15 (2018). https://doi.org/10.1186/s12915-018-0524-5.
    2. Jia, H., Kai, L., Heymann, M., Garcia-Soriano, D.A., Härtel, T., Schwille, P.: Light-Induced Printing of Protein Structures on Membranes in Vitro ̈. Nano Letters. 18, 7133--7140 (2018). https://doi.org/10.1021/acs.nanolett.8b03187.
    3. Gicquel, Y., Schubert, R., Kapis, S., Bourenkov, G., Schneider, T., Perbandt, M., Betzel, C., Chapman, H.N., Heymann, M.: Microfluidic chips for in situ crystal X-ray diffraction and in situ dynamic light scattering for serial crystallography. Journal of Visualized Experiments. (2018). https://doi.org/10.3791/57133.
    4. Haiyang, J., Heymann, M., Bernhard, F., Schwille, P., Kai, L.: Cell free protein synthesis in micro compartments: building a minimal cell from bio bricks. submitted. (2018).
    5. Wiedorn, M.O., Awel, S., Morgan, A.J., Ayyer, K., Gevorkov, Y., Fleckenstein, H., Roth, N., Adriano, L., Bean, R., Beyerlein, K.R., Chen, J., Coe, J., Cruz-Mazo, F., Ekeberg, T., Graceffa, R., Heymann, M., Oberthür, D., Samanta, A.K., Sierra, R.G., Stan, C.A., Yefanov, O., Rompotis, D., Correa, J., Erk, B., Treusch, R., Schulz, J., Hogue, B.G., Ganan-Calvo, A.M., Fromme, P., Küpper, J., Rode, A.V., Bajt, S., Kirian, R.A., Chapman, H.N.: Rapid sample delivery for megahertz serial crystallography at X-ray FELs. IUCrJ. 5, 574--584 (2018). https://doi.org/10.1107/S2052252518008369.
    6. Niederauer, C., Blumhardt, P., Mücksch, J., Heymann, M., Lambacher, A., Schwille, P.: Direct characterization of the evanescent field in objective-type total internal reflection fluorescence microscopy. Optics Express. 26, 574--582 (2018). https://doi.org/10.1364/OE.26.020492.
    7. Wang, Q., Taschner, M., Ganzinger, K.A., Kelley, C., Villasenor, A., Heymann, M., Schwille, P., Lorentzen, E., Mizuno, N.: Membrane association and remodeling by intraflagellar transport protein IFT172. Nature Communications. 9, 1--13 (2018). https://doi.org/10.1038/s41467-018-07037-9.
    8. Wiedorn, M.O., Oberthür, D., Bean, R., Schubert, R., Werner, N., Abbey, B., Aepfelbacher, M., Adriano, L., Allahgholi, A., Al-Qudami, N., Andreasson, J., Aplin, S., Awel, S., Ayyer, K., Bajt, S., Barák, I., Bari, S., Bielecki, J., Botha, S., Boukhelef, D., Brehm, W., Brockhauser, S., Cheviakov, I., Coleman, M.A., Cruz-Mazo, F., Danilevski, C., Darmanin, C., Doak, R.B., Domaracky, M., Dörner, K., Du, Y., Fangohr, H., Fleckenstein, H., Frank, M., Fromme, P., Gañán-Calvo, A.M., Gevorkov, Y., Giewekemeyer, K., Ginn, H.M., Graafsma, H., Graceffa, R., Greiffenberg, D., Gumprecht, L., Göttlicher, P., Hajdu, J., Hauf, S., Heymann, M., Holmes, S., Horke, D.A., Hunter, M.S., Imlau, S., Kaukher, A., Kim, Y., Klyuev, A., Knoska, J., Kobe, B., Kuhn, M., Kupitz, C., Küpper, J., Lahey-Rudolph, J.M., Laurus, T., Le Cong, K., Letrun, R., Xavier, P.L., Maia, L., Maia, F.R.N.C., Mariani, V., Messerschmidt, M., Metz, M., Mezza, D., Michelat, T., Mills, G., Monteiro, D.C.F., Morgan, A., Mühlig, K., Munke, A., Münnich, A., Nette, J., Nugent, K.A., Nuguid, T., Orville, A.M., Pandey, S., Pena, G., Villanueva-Perez, P., Poehlsen, J., Previtali, G., Redecke, L., Riekehr, W.M., Rohde, H., Round, A., Safenreiter, T., Sarrou, I., Sato, T., Schmidt, M., Schmitt, B., Schönherr, R., Schulz, J., Sellberg, J.A., Seibert, M.M., Seuring, C., Shelby, M.L., Shoeman, R.L., Sikorski, M., Silenzi, A., Stan, C.A., Shi, X., Stern, S., Sztuk-Dambietz, J., Szuba, J., Tolstikova, A., Trebbin, M., Trunk, U., Vagovic, P., Ve, T., Weinhausen, B., White, T.A., Wrona, K., Xu, C., Yefanov, O., Zatsepin, N., Zhang, J., Perbandt, M., Mancuso, A.P., Betzel, C., Chapman, H., Barty, A.: Megahertz serial crystallography. Nature Communications. 9, 4025 (2018). https://doi.org/10.1038/s41467-018-06156-7.
    9. Ramm, B., Glock, P., Mücksch, J., García soriano, D.A., Heymann, M., Schwille, P.: The MinDE system is a generic spatial cue for membrane protein distribution in vitro. Nature Communications. 9, 3942 (2018). https://doi.org/10.1038/s41467-018-06310-1.
    10. Litschel, T., Ramm, B., Maas, R., Heymann, M., Schwille, P.: Beating Vesicles: Encapsulated Protein Oscillations Cause Dynamic Membrane Deformations. Angewandte Chemie International Edition. 57, 16286--16290 (2018). https://doi.org/10.1002/anie.201808750.
    11. Litschel, T., Ganzinger, K.A., Movinkel, T., Heymann, M., Robinson, T., Mutschler, H., Schwille, P.: Freeze-thaw cycles induce content exchange between cell-sized lipid vesicles Freeze-thaw cycles induce content exchange between cell-sized lipid vesicles. New Journal of Physics. 055008, 11 (2018). https://doi.org/10.1088/1367-2630/aabb96.
  4. 2017

    1. Rambach, R.W., Linder, K., Heymann, M., Franke, T.: Droplet trapping and fast acoustic release in a multi-height device with steady-state flow. Lab on a Chip. 17, (2017). https://doi.org/10.1039/c7lc00378a.
    2. Wiedorn, M.O., Awel, S., Morgan, A.J., Barthelmess, M., Bean, R., Beyerlein, K.R., Chavas, L.M.G., Eckerskorn, N., Fleckenstein, H., Heymann, M., Horke, D.A., Knoska, J., Mariani, V., Oberthür, D., Roth, N., Yefanov, O., Barty, A., Bajt, S., Küpper, J., Rode, A.V., Kirian, R.A., Chapman, H.N.: Post-sample aperture for low background diffraction experiments at X-ray free-electron lasers. Journal of Synchrotron Radiation. 24, (2017). https://doi.org/10.1107/S1600577517011961.
    3. Wojtas, D.H., Ayyer, K., Liang, M., Mossou, E., Romoli, F., Seuring, C., Beyerlein, K.R., Bean, R.J., Morgan, A.J., Oberthuer, D., Fleckenstein, H., Heymann, M., Gati, C., Yefanov, O.M., Barthelmess, M., Ornithopoulou, E., Galli, L., Xavier, P.L., Ling, W.L., Frank, M., Yoon, C.H., White, T.A., Bajt, S., Mitraki, A., Boutet, S., Aquila, A., Barty, A., Forsyth, T., Chapman, H.N., Millane, R.P.: Analysis of XFEL serial diffraction data from individual crystalline fibrils. IUCrJ. 4, 795--811 (2017). https://doi.org/10.1107/S2052252517014324.
    4. Beyerlein, K.R., Dierksmeyer, D., Mariani, V., Kuhn, M., Sarrou, I., Ottaviano, A., Awel, S., Knoska, J., Fuglerud, S., Jönsson, O., Stern, S., Wiedorn, M.O., Yefanov, O., Adriano, L., Bean, R., Burkhardt, A., Fischer, P., Heymann, M., Horke, D.A., Jungnickel, K.E.J., Kovaleva, E., Lorbeer, O., Metz, M., Meyer, J., Morgan, A., Pande, K., Panneerselvam, S., Seuring, C., Tolstikova, A., Lieske, J., Aplin, S., Roessle, M., White, T.A., Chapman, H.N., Meents, A., Oberthuer, D.: Mix-and-diffuse serial synchrotron crystallography. IUCrJ. 4, (2017). https://doi.org/10.1107/S2052252517013124.
    5. Oberthuer, D., Knoska, J., Wiedorn, M.O., Beyerlein, K.R., Bushnell, D.A., Kovaleva, E.G., Heymann, M., Gumprecht, L., Kirian, R.A., Barty, A., Mariani, V., Tolstikova, A., Adriano, L., Awel, S., Barthelmess, M., Dörner, K., Xavier, P.L., Yefanov, O.M., James, D., Nelson, G., Wang, D., Calvey, G., Chen, Y., Schmidt, A., Szczepek, M., Frielingsdorf, S., Lenz, O., Snell, E., Robinson, P.J., Sarler, B., Belsak, G., Macek, M., Wilde, F., Aquila, A., Boutet, S., Liang, M., Hunter, M.S., Scheerer, P., Lipscomb, J.D., Weierstall, U., Kornberg, R.D., Spence, J.C.H., Pollack, L., Chapman, H.N., Bajt, S.: Double-flow focused liquid injector for efficient serial femtosecond crystallography. Scientific Reports. 7, 44628 (2017). https://doi.org/10.1038/srep44628.
    6. Aghvami, S.A., Opathalage, A., Zhang, Z.K., Ludwig, M., Heymann, M., Norton, M., Wilkins, N., Fraden, S.: Rapid prototyping of cyclic olefin copolymer (COC) microfluidic devices. Sensors and Actuators, B: Chemical. 247, (2017). https://doi.org/10.1016/j.snb.2017.03.023.
    7. Jia, H., Heymann, M., Bernhard, F., Schwille, P., Kai, L.: Cell-free protein synthesis in micro compartments: Building a minimal cell from biobricks. New Biotechnology. (2017). https://doi.org/10.1016/j.nbt.2017.06.014.
  5. 2016

    1. Wang, A.L.L., Gold, J.M.M., Tompkins, N., Heymann, M., Harrington, K.I.I., Fraden, S.: Configurable NOR gate arrays from. European Physical Journal. 225, 211--227 (2016). https://doi.org/10.1140/epjst/e2016-02622-y.
    2. Nelson, G., Kirian, R.A.R.A., Weierstall, U., Zatsepin, N.A., Faragó, T., Baumbach, T., Wilde, F., Niesler, F.B.P., Zimmer, B., Ishigami, I., Hikita, M., Bajt, S., Yeh, S.-R., Rousseau, D.L., Chapman, H.N., Spence, J.C.H., Heymann, M.: Three-dimensional-printed gas dynamic virtual nozzles for x-ray laser sample delivery. Optics Express. 24, 11515--11530 (2016). https://doi.org/10.1364/OE.24.011515.
    3. Schubert, R., Kapis, S., Gicquel, Y., Bourenkov, G., Schneider, T.R.T.R., Heymann, M., Betzel, C., Perbandt, M.: A multicrystal diffraction data-collection approach for studying structural dynamics with millisecond temporal resolution. IUCrJ. 3, 393--401 (2016). https://doi.org/10.1107/S2052252516016304.
  6. 2015

    1. Feld, G.K., Heymann, M., Benner, W.H., Pardini, T., Tsai, C.-J., Boutet, S., Coleman, M.A., Hunter, M.S., Li, X.-D.X., Messerschmidt, M., Opthalage, A., Pedrini, B., Williams, G.J., Krantz, B.A., Fraden, S., Hau-Riege, S.P., Evans, J.E., Segelke, B.W., Frank, M., Opathalage, A., Pedrini, B., Williams, G.J., Krantz, B.A., Fraden, S., Hau-Riege, S.P., Evans, J.E., Segelke, B.W., Frank, M.: Low- Z polymer sample supports for fixed-target serial femtosecond X-ray crystallography. Journal of Applied Crystallography. 48, 1072--1079 (2015). https://doi.org/10.1107/S1600576715010493.
    2. Tompkins, N., Cambria, M.C., Wang, A.L., Heymann, M., Fraden, S.: Creation and perturbation of planar networks of chemical oscillators. Chaos. 25, 064611 (2015). https://doi.org/10.1063/1.4922056.
    3. Chen, D.T.N., Heymann, M., Fraden, S., Nicastro, D., Dogic, Z.: ATP Consumption of Eukaryotic Flagella Measured at a Single-Cell Level. Biophysical Journal. 109, 2562--2573 (2015). https://doi.org/10.1016/j.bpj.2015.11.003.
    4. Beyerlein, K.R., Adriano, L., Heymann, M., Kirian, R.A., Knoska, J., Wilde, F., Chapman, H.N., Knoska, J., Wilde, F., Chapman, H.N., Bajt, S.: Ceramic micro-injection molded nozzles for serial femtosecond crystallography sample delivery. Review of Scientific Instruments. 86, 125104 (2015). https://doi.org/10.1063/1.4936843.
    5. Kirian, R.A., Awel, S., Eckerskorn, N., Fleckenstein, H., Wiedorn, M., Adriano, L., Bajt, S., Barthelmess, M., Bean, R., Beyerlein, K.R., Chavas, L.M.G., Domaracky, M., Heymann, M., Horke, D. a., Knoska, J., Metz, M., Morgan, A., Oberthuer, D., Roth, N., Sato, T., Xavier, P.L., Yefanov, O., Rode, a V., Küpper, J., Chapman, H.N.: Simple convergent-nozzle aerosol injector for single-particle diffractive imaging with X-ray free-electron lasers. Structural Dynamics. 2, 041717 (2015). https://doi.org/10.1063/1.4922648.
    6. Nogly, P., James, D., Wang, D., White, T. a., Zatsepin, N., Shilova, A., Nelson, G., Liu, H., Johansson, L., Heymann, M., Jaeger, K., Metz, M., Wickstrand, C., Wu, W., B\aath, P., Berntsen, P., Oberthuer, D., Panneels, V., Cherezov, V., Chapman, H., Schertler, G., Neutze, R., Spence, J., Moraes, I., Burghammer, M., Standfuss, J., Weierstall, U.: Lipidic cubic phase serial millisecond crystallography using synchrotron radiation. IUCrJ. 2, 168--176 (2015). https://doi.org/10.1107/S2052252514026487.
  7. 2014

    1. Tompkins, N., Li, N., Girabawe, C., Heymann, M., Ermentrout, G.B., Epstein, I.R., Fraden, S.: Testing Turing’s theory of morphogenesis in chemical cells. PNAS. 111, 4397--402 (2014). https://doi.org/10.1073/pnas.1322005111.
    2. Akella, S.V., Mowitz, A., Heymann, M., Fraden, S.: Emulsion-Based Technique To Measure Protein Crystal Nucleation Rates of Lysozyme. Crystal Growth and Design. 14, 4487--4509 (2014). https://doi.org/10.1021/cg500562r.
    3. Heymann, M., Opthalage, A., Wierman, J.L., Akella, S., Szebenyi, D.M.E., Gruner, S.M., Fraden, S.: Room-temperature serial crystallography using a kinetically optimized microfluidic device for protein crystallization and on-chip X-ray diffraction. IUCrJ. 1, 349--360 (2014). https://doi.org/10.1107/S2052252514016960.
    4. Kornreich, M., Heymann, M., Fraden, S., Beck, R.: Cross polarization compatible dialysis chip. Lab on a chip. 14, 3700--4 (2014). https://doi.org/10.1039/c4lc00600c.
    5. Tenboer, J., Basu, S., Zatsepin, N., Pande, K., Milathianaki, D., Frank, M., Hunter, M., Boutet, S., Williams, G.J., Koglin, J.E., Oberthuer, D., Heymann, M., Kupitz, C., Conrad, C., Coe, J., Roy-Chowdhury, S., Weierstall, U., James, D., Wang, D., Grant, T., Barty, A., Yefanov, O., Scales, J., Gati, C., Seuring, C., Srajer, V., Henning, R., Schwander, P., Fromme, R., Ourmazd, A., Moffat, K., Van Thor, J.J., Spence, J.C.H., Fromme, P., Chapman, H.N., Schmidt, M.: Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein. Science. 346, 1242--1246 (2014). https://doi.org/10.1126/science.1259357.
  8. 2013

    1. Heymann, M., Fraden, S., Kim, D.: Multi-Height Precision Alignment With Selectively Developed Alignment Marks. Journal of Microelectromechanical Systems. 23, 1--4 (2013). https://doi.org/10.1109/JMEMS.2013.2279231.
  9. 2012

    1. Sanchez, T., Chen, D.T.N., DeCamp, S.J., Heymann, M., Dogic, Z.: Spontaneous motion in hierarchically assembled active matter. Nature. 491, 431--4 (2012). https://doi.org/10.1038/nature11591.
  10. 2010

    1. Heymann, M., Harrington, K.I., Pollack, J., Fraden, S.: En Route to Signal Inversion in Chemical Computing. Proceedings of Artificial Life XII. 166--167 (2010).
    2. Romanowsky, M.B., Heymann, M., Abate, A.R., Krummel, A.T., Fraden, S., Weitz, D.A.: Functional patterning of PDMS microfluidic devices using integrated chemo-masks. Lab on a chip. 10, 1521--4 (2010). https://doi.org/10.1039/c004050a.

Kontakt

Michael Heymann
Jun.Prof. Dr.

Michael Heymann

Abteilungsleiter

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