This video describes how to use TIRF microscopy to track single ion channels and determine their activity in supported lipid membranes, thereby defining the interplay between lateral membrane movement and channel function. It describes how to prepare the membranes, record the data, and analyze the results.
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For reference, see: Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using TIRF Microscopy. Wang, S. & Nussberger, S. J. Vis. Exp. 192: 1-21, e64970, doi 10.3791/64970 (2023)